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Disclosure of interest: No disclosures E-Posters - RECOVERY, REHABILITATION AND OUTCOME 03.00 - RECOVERY, REHABILITATION AND OUTCOME - 03.02 - LIFE AFTER STROKE P2275 - ESOC25-1701 PREVALENCE OF RYANODINE ANTIBODIES IN STROKE PATIENTS WITH POST-STROKE FATIGUE (PSF): AN OBSERVATIONAL ANALYSIS Dr Karthika Pt, Vivek Nambiar 1 , Dr Sudheeran Kannoth 1 , Divyasree Chandran 1 Amrita Institute of Medical Sciences and Research Centre, Kochi, India Background and Aims: Post-stroke fatigue (PSF) is a debilitating sequela of stroke involving central and peripheral mechanisms

SWISS-MODEL: homology modelling of protein structures and complexes

At the molecular level, 5-amino-1MQ functions as a competitive inhibitor of NNMT, demonstrating remarkable potency with an IC of 1.2 0.1 M under standard assay conditions (50 M SAM, 100 M nicotinic acid). This represents a dramatic 10-fold improvement over the parent compound 1-methylquinolinium, achieved through strategic amino group substitution that enhances binding affinity to the NNMT active site. The compound's mechanism centers on preventing the methylation of nicotinamide to 1-methylnicotinamide (1-MNA), thereby preserving nicotinamide for recycling back to NAD+ through the salvage pathway. This intervention effectively blocks what researchers have termed the "NNMT metabolic drain" a process that simultaneously depletes NAD+ precursors and consumes cellular methylation capacity

But the functional result is the same: a brain losing capacity over time, with current medicine offering few effective interventions to slow or reverse the process

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