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After an additional 24 h, all media were aspirated and replaced with either DMEM or DMEM supplemented with 50 M deferoxamine (DFO)
The mechanism may be that CB-839 and AC220 synergistically deplete glutathione, induce the production of mitochondrial ROS, and lead to apoptosis

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Canto C, Jiang LQ, Deshmukh AS, Mataki C, Coste A, Lagouge M, et al

The high mineral content and bioactive compounds may affect hormonal balance and fetal development
