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Fixed ammonia is subsequently assimilated into glutamate through the glutamine synthetase/glutamate:2-oxoglutarate aminotransferase (GS-GOGAT) pathway, and the glutamate thus formed is then used for cell growth and the renewal of nitrogenase to maintain diazotrophy 11,12

These results indicated that folate supplementation during pregnancy could regulate the gut microbiota of male offspring and improve gut barrier integrity, and folate inhibited the expression of the TLR4/NFB pathway in the liver, thereby ameliorating lipid metabolic disorders and alleviating hepatic steatosis

Baseline measurements included the number of days the consultant had been on the unit, their decision fatigue scale (DFS) score 1 and their reaction times

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