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The method of calculating binding constants was to measure the absorbance (minus the absorbance of the buffer) of 100 L of each compound by itself diluted by 100 L of buffer at a given wavelength (in this case at 200 nm) at the range of concentrations utilized in the experiments

This may be due to the fact that the control sample (turmeric without extraction) contained more amorphous regions than turmeric subjected to extraction using SFE + UAE

The treatment of hepatotoxicity is dependent upon the causative agent, the degree of liver dysfunction and the age and general health of the individual

The reaction mixture is then centrifuged and washed until the precipitate is free from sulphates

The liver naturally produces it