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The combined sample (200 g) was separated into 8 fractions by Pierce High pH Reverse-Phase Peptide Fractionation kit (Thermo Scientific) with an extra wash before separation to remove extra labels 17

The preservation of peptide stability has been observed for at least 28 days after reconstitution.[15] Peptides stored in BAC Water maintained 98% activity in assays up to 14 days post-reconstitution.[16] 2.3 Microbial Control & Aseptic Handling Benzyl alcohols bacteriostatic action has been well documented in pharmaceutical formulations, especially in multi-use vials, which ensures that peptides do not become contaminated during the study period.[17][18] Multiple use vials of BAC Water for research have shown reduced microbial load, even after repeated sampling.[19] 2.4 Storage & Stability Considerations While BAC Water extends the shelf life of reconstituted peptides, its effectiveness relies on proper storage conditions

503A as bulk drug substances that may be used in traditional patient-specific compounding