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Cells were incubated with serum-free media for 24 h prior to each experiment and seeded at 1 10 6 cells/dish in 100 mm culture dishes for western blotting and 3 10 4 cells/well in 24-well- culture plates for immunofluorescence

This is because menopause brings hormonal changes like lower estrogen levels, which lead to a range of effects that include weight gain for many women

Eligible beneficiaries pay a flat $50 monthly copayment for covered medications

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