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Step 8: Store Appropriately Immediately transfer aliquots to the appropriate storage condition: Long-term (up to 3 months): -80C freezer, protected from light Short-term (up to 7 days): 4C refrigerator, protected from light Working dilutions: Prepare fresh from frozen aliquots on the day of the experiment Avoid storing reconstituted peptide at -20C for long periods, as the frost-free cycling in most -20C freezers creates repeated low-level temperature fluctuations that degrade peptides over time

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