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The R311C missense mutation maps to a highly-conserved feature called the Histidine-Arginine-Aspartate (HRD) motif located within the catalytic kinase domain of CaMKK2 (Fig

[DOI] [PubMed] [Google Scholar] 85.Coskun T., Sloop K.W., Loghin C., Alsina-Fernandez J., Urva S., Bokvist K.B., Cui X., Briere D.A., Cabrera O., Roell W.C., et al

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28,36 Theoretically, the substitution of Gly 22 with Ala would make the -helix more stable, but increments in the affinity and activity were not found