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Subsequently, these two groups independently reported that the synthetic GLP-1 peptide acted as a potent stimulator of insulin secretion in isolated perfused rat pancreas (24) and isolated perfused pig pancreas (23), respectively, thus implicating GLP-1 as the second incretin

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12-hour light and 12-hour dark cycles) with free access to standard chow (63) or high-cholesterol diet when relevant

There was a one-week interval between each testing condition (drug or vehicle) to allow for any potential carryover effects and maintain experimental integrity

After SLZ injection, the number of positive cells in the submucosa dramatically reduced (Fig