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Denatured samples (100C for 5 min) were separated by SDS electrophoresis, blotted onto a nitrocellulose membrane (Amersham Protram 0.2 m PC, GE Healthcare Life Sciences, United States), and incubated with specific polyclonal antibody at the following concentrations: anti-AOX (Agrisera, Sweden) 1:100, anti-NgDJ-1 1 1:50, and anti-NfNDH-2 1:1,000

The logistic regression of this study thereby refers to all tooth surfaces

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