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Perrott J, et al

Quantitative reverse transcription polymerase chain reaction (qRTPCR) was performed on a real-time PCR system (ABI7500, Bio-Rad, USA) using ChamQ Universal SYBR qPCR Master Mix (catalog number: Q711-03, Vazyme, Nanjing, China)

Lactate is formed, reductively, from pyruvate as the final reaction of glycolysis, diverting pyruvate from oxidation to acetyl-CoA which is the first step in oxidative phosphorylation

3 Results 3.1 Dedicated library of potential yeast xenobiotics transporters For expression, we selected transporter proteins from secondary active transporter superfamilies known to transport drugs and natural compounds