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Before fluorescence-activated cell sorting (FACS) analysis, BALF samples were collected, washed and fixed with 4% paraformaldehyde, and lysis of red blood cells with ammonium-chloride-potassium (ACK) lysing buffer was performed for blood samples according to the manufacturers instructions

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BPC 157 is a synthetic research peptide used in laboratory and preclinical studies to explore peptide driven cellular signaling, tissue integrity, and adaptive biological responses