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With the use of a Gibson Assembly (NEBuilder HiFi DNA Assembly), it was cloned into a p2Tol-based vector harboring puromycin resistance (Addgene category catalog number 714859)

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That is, instead of being confined to a single pathway, it possesses a multimodal action profile capable of promoting tissue healing, attenuating neuroinflammation, modulating key comorbidities like obesity and cancer, and interacting with endocrine signaling
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