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These uncertainties manifest themselves also in the cross validation through a significantly increased standard deviation ((slope) = 0.12

Single- or double-antigen labeling of formalin-fixed and paraffin-embedded 4-m-thick sections by immunocytochemistry (ICC) or immunohistochemistry (IHC) included the following steps: deparaffinization, rehydratation, boiling in EDTA buffer (1 mM EDTA, 0.05% Tween 20, pH 8.0) for 15 min, 1% H 2 O 2 incubation for 15 min and blocking in SuperBlock solution (ScyTek Laboratories, Logan, UT) for 30 min, followed by primary antibody application

The interaction of gliadin and its immunomodulatory fragments with chemokine CXC receptor 3 on IECs induces zonulin release and downstream disassembly of TJs which has been reported previously among coeliac patients (Lammers et al

This is particularly concerning with KPV (NF-kB suppression) and thymosin alpha-1 (immune regulation)

Peptide quality: This is a significant variable