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doi: 10.2337/dc22-1148 [DOI] [PubMed] [Google Scholar] 26
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The assay involved the sequential addition of 2 mM malate, 5 mM glutamate, and 10 mM pyruvate (complex I leak respiration), 5 mM ADP (complex I oxidative phosphorylation), 10 mM succinate (complex I + II oxidative phosphorylation), 2.5 M oligomycin (complex I + II leak respiration), 0.5 M FCCP (maximal respiration), and 2.5 M antimycin A (non-mitochondrial respiration)
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